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Matrix Science mascot algorithm v 2 4 2
Mascot Algorithm V 2 4 2, supplied by Matrix Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mascot+algorithm/distiller+mascot/pm42086780-508-27-31
Average 86 stars, based on 1 article reviews
mascot algorithm v 2 4 2 - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Complement and Coagulation Cascades Pathway was Inactivated in HIV-Associated Colorectal Cancer: Results from a Proteomics Study
Article Snippet: Protein identification was performed via the Mascot algorithm (v2.3.02; Matrix Science) against the UniProt Human reference proteome database (release 2022.2.8, 9606 entries).

Article Title: Phosphoproteomics identifies Rho-kinase-associated phospho-signaling and autophagy-associated alterations in the medial prefrontal cortex of a schizophrenia-related mouse model.
Article Snippet: Raw LC-MS/MS data were processed using Proteome Discoverer (version 1.4, Thermo Fisher Scientific) and searched with the MASCOT algorithm (version 2.8.0, Matrix Science Inc., Boston, MA) against the UniProt Mus musculus database (release 2023_01).

Modification:

Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes
Article Snippet: .. The mgf files were then run against the Acetonema longum DSM 6540 Uniprot database using the Mascot algorithm (Matrix Sciences; version 2.7), specifying trypsin digestion with a maximum of 1 missed cleavage, peptide charge of ≥2, cysteine carbamidomethylation as the fixed modification, methionine oxidation as the variable modification, and mass error tolerance of 10 ppm (0.6 Da for fragmented ions). .. Dormant or germinating endospore pellets were solubilized in 60 μl 1× Laemmli SDS-PAGE dye, boiled at 95 °C for 10 min, subjected to bead-beating at 5 m/s for 2 × 30 s with 0.1 mm Zirconia/Silica beads using FastPrep-24 (MP Biomedicals), then boiled again at 95 °C for 10 min.

Derivative Assay:

Article Title: T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies.
Article Snippet: .. Peptide identification of CTL derived WT exosomes analyzed with the Orbitrap Velos setup was performed using the MASCOT algorithm (Matrix Science) and TF Proteome Discoverer 1.4, searching the SwissProt database against mouse proteins (17040 mus musculus entries). .. Peptide identification of CTL derived WT exosomes analyzed with the Orbitrap Velos setup was performed using the MASCOT algorithm (Matrix Science) and TF Proteome Discoverer 1.4, searching the SwissProt database against mouse proteins (17040 mus musculus entries).

Article Title: T‐Cell‐Derived Exosomes From Multi Core Granules Exhibit Superior Caspase‐3‐Mediated Tumor‐Suppressive Activity Compared to Those From Multivesicular Bodies
Article Snippet: .. Peptide identification of CTL derived WT exosomes analyzed with the Orbitrap Velos setup was performed using the MASCOT algorithm (Matrix Science) and TF Proteome Discoverer 1.4, searching the SwissProt database against mouse proteins (17040 mus musculus entries). .. Peptide identification of CTL derived WT exosomes analyzed with the Orbitrap Velos setup was performed using the MASCOT algorithm (Matrix Science) and TF Proteome Discoverer 1.4, searching the SwissProt database against mouse proteins (17040 mus musculus entries).

Software:

Article Title: Rapid purification of brain protein complexes containing active and inactive forms of the G protein Gαo.
Article Snippet: .. Tandem mass spectra were extracted by Proteome Discoverer software (version 2.2.0.388, Thermo Scientific) and searched in-house using the Mascot algorithm (version 2.6.1, Matrix Science). ..

Article Title: Actn4 Links Inactive Integrin α5 With Actin in Zebrafish Somites
Article Snippet: Peptides were separated at 37 °C using a Waters ACQUITY UPLC M-Class Peptide BEH C18 Column (130 Å, 1.7 μm, 75 μm × 250 mm) and eluted at 300 nl/min with the following gradient: 3% buffer B at initial conditions; 5% B at 2 min; 25% B at 140 min; 40% B at 165 min; 90% B at 170 min; 90% B at 180 min; return to initial conditions at 182 min. MS was acquired in profile mode over the 300-1700 m/z range using 1 microscan, 70,000 resolution, AGC target of 3E6, and a maximum injection time of 45 ms. Data-dependent acquisition (DDA) MS/MS were acquired in centroid mode on the top 20 precursors per MS scan using 1 microscan, 17,500 resolution, AGC target of 1E5, maximum injection time of 100 ms, and an isolation window of 1.7 m/z. .. Tandem mass spectra were extracted by Proteome Discoverer software (version 2.2.0.388, Thermo Scientific) and searched in-house using the Mascot algorithm (version 2.6.1, Matrix Science). ..

Tandem Mass Spectroscopy:

Article Title: Screening method for client protein-protecting protein, and physiologically active protein-stabilizing protein and pharmaceutical composition comprising said protein
Article Snippet: LTQ-Orbitrap Velos mass spectrometer (manufactured by Thermo Fisher Scientific) coupled with Dina-2A nanoflow LC system (manufactured by KYA Technologies) was used to perform nano liquid chromatography tandem mass spectrometry (nano LC-MS/MS) analysis according to a routine method. .. In order to analyze the relative amino acid frequency of the identified heat-soluble proteins, the MS/MS signals were processed against the UniProt human proteome (Homo sapiens; UP000005640) and Drosophila proteome (Drosophila melanogaster; UP000000803) using the Mascot algorithm (version 2.5.1; Matrix Science). ..



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